Daniel Vik

4 Questions 9 Answers 0 Followers

Questions related from Daniel Vik

Is it possible to measure both peptides and metabolites on the same triplequad?

Hi I am working in a lab which does extensive metabolite analysis using triplequad (Bruker EVOQ Elite) and QTOF (Bruker Compact) instruments. We are about to set up a method for measuring peptides...

20 April 2015 3,140 6 View

Interference of GFP tag on protein structure/function?

GFP is a commonly used tag for protein studies. However, I often hear that because GFP is a bulky (~20 kD) protein it can interfere with the system being studied. Does anyone know if this have...

13 May 2013 9,723 8 View

Co-IP elution as a smear?

I am trying to do Co-IP using Pierce Crosslink Immunoprecipitation Kit. However, something is puzzling me. When I do the elution and subsequently visualize by western blot, I quite often get...

19 April 2013 3,482 10 View

How do you avoid denaturing antibodies when doing IP under denaturing conditions?

I am currently trying to precipitate a protein for which I have an antibody targeting a peptide epitope. However, so far it seems likely that the epitope is buried. I have heard that it is...

21 February 2013 3,281 3 View