Daniel Stephens

2 Questions 4 Answers 0 Followers

Questions related from Daniel Stephens

Order of minigene-GFP construct for IVT and electroporation for human dendritic cells?

We are developing a GFP construct to use as a marker for successful electroporation of a minigene into human dendritic cells. the purpose is to use the dendritic cells as potential targets for t...

29 January 2014 4,455 1 View

What is the best isotype for assessing background for cell surface activation/phenotyping markers by FACS?

I'm doing some extensive phenotyping by FACS of human lymphocyte populations. I'm looking at a number of markers- 41BB CD137 OX40 CD134 PD1 CTLA4 TIM3.... For many of these markers the isotype...

20 January 2014 8,157 3 View