Daniel Kumazawa Morais

3 Questions 6 Answers 0 Followers

Questions related from Daniel Kumazawa Morais

Is there anyone who works with Gram positive and Gram negative bacteria transformed with Green Fluorescent Protein (GFP)?

I'm developing a method for DNA extraction from soil, and these transformed bacteria would be great to use as a standard for DNA/cell quantification. Could anyone send me a sample of these or put...

23 April 2013 3,498 0 View

Which is the best way to evaluate the efficiency of DNA extraction from soil samples?

I will extract DNA from different soil treatments for shotgun metagenomic analysis. And I'm worried about the different amounts of DNA extracted in each treatment. How can I measure the efficiency...

26 March 2013 6,268 5 View

How many lanes of an illumina sequencer flow cell would be necessary to study a soil metagenomics? Using dna shotgun.

I thought in a paired-end sistem that gives me reads of 250 bp making contigs of 500 bp.

04 October 2012 9,909 9 View