I have been trying to clone a 5.2 kb gene. I used the NEB Gibson Assembly Mix but still, without success. Any suggestion please?

I fragmented the gene into 2 pieces but I could not PCR-amplify the fragments from (cDNA). I then fragmented it into 5 pieces and amplified the fragments but the gibson assembly did not work (just...

11 June 2021 9,057 6 View