Daniel Belcher

2 Questions 4 Answers 0 Followers

Questions related from Daniel Belcher

What is the optimal blocking buffer (Milk vs BSA) for unpurified antiserum antibodies in western blots?

We received a limited amount of an antiserum antibody as a gift from another lab and are trying to avoid having to purify it. Our initial titration blot with blocking in 5% milk successfully...

25 April 2024 9,373 2 View

Why are my Western Blot transfers leaving so much protein behind in the gel?

*Before you comment with any of the generic troubleshooting advice from the front page of Google, just know I've already tried all that* No matter how I alter my conditions for wet tank transfer,...

31 October 2022 6,318 6 View