Dan Liu

3 Questions 1 Answers 0 Followers

Questions related from Dan Liu

Any suggestions for the primers for real-time PCR using mouse genomic DNA as template?

We have benn trying to test the relative copy number of retriviral insertion into the mouse genome, any suggestions of the primers in this case? Theoretically I suppose two copies of the normal...

10 October 2016 9,446 0 View

How can I establish MEFs as a cell line?

As the title says, does it work for serial passage that MEFs transform and become immortal ?

03 March 2016 9,345 1 View

How can I digest cells into single cells that are overgrown, like in my case, infected MEFs after culture over a week?

I have infected MEFs for induction in vitro, but after culture over a week, I found the MEFs form a compact over the petri dish, the normal trypinisation doesn't work. I also tried collogenase IV,...

12 December 2015 8,638 5 View