Dan Jones

2 Questions 4 Answers 0 Followers

Questions related from Dan Jones

How long do the transfection complexes (transfection reagent + DNA) remain in cell culture medium?

For example, in some experiments I transfect cells with plasmid DNA to produce retroviruses pseudotyped with other viral envelope proteins. I am using X-tremeGENE HP, which does not require the...

11 November 2013 2,438 2 View

What would be the suitable storage conditions for X-gal dissolved in DMSO?

The data sheet provided with my X-gal powder is stunningly cryptic. It mentions that X-gal stock solutions should preferably be stored at -20 in a glass container, since DMF (which can be used to...

11 November 2013 7,006 1 View