Dan Ryder

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Questions related from Dan Ryder

Has anyone successfully expressed and purified a trimeric protein complex in mammalian tissue culture cells for analysis via crystalization?

I am trying to find a technique where someone has successfully expressed and purified a protein complex (three different proteins) to then be used in x-ray crystalography.

30 May 2013 4,684 2 View

I would like to get someone’s opinion on a ubiquitin activity assay/kit.

Has anyone used the following assays to measure ubiquitin activity? If so, please let me know what you think. 1.) Enzo Life Sci (Ubiquitin activating kit, BML-UW0400A-0001) (or) 2.) Boston Biochem...

14 January 2013 1,089 4 View