Dan Ryder

6 Questions 13 Answers 0 Followers

Questions related from Dan Ryder

Has anyone successfully expressed and purified a trimeric protein complex in mammalian tissue culture cells for analysis via crystalization?

I am trying to find a technique where someone has successfully expressed and purified a protein complex (three different proteins) to then be used in x-ray crystalography.

30 May 2013 4,733 2 View

I would like to get someone’s opinion on a ubiquitin activity assay/kit.

Has anyone used the following assays to measure ubiquitin activity? If so, please let me know what you think. 1.) Enzo Life Sci (Ubiquitin activating kit, BML-UW0400A-0001) (or) 2.) Boston Biochem...

14 January 2013 1,138 4 View

Is it possible to see in vitro or in vivo ubiquitin ligase activity after overexpression of just the Hect domain of the E3 ubiquitin ligase Hectd2?

I would like to assay for the effect of Hectd2 activity on my protein of interest but the actual Hectd2 protein is too big. I would like to know if I can express the Hect domain only to test my...

01 October 2012 1,652 12 View

Is there an age limit I should abide by if I want to isolate adult skeletal muscle progenitor cells from rats?

I would like to learn how to isolate adult skeletal muscle progenitors from male SD rats and eventually differentiate them into myotubes for further experimentation.

19 September 2012 4,532 7 View

What is the best method to deliver plasmid DNA to freshly isolated adult skeletal muscle (rat) progenitor cells?

I would like to delivery plasmid DNA (~10kb) to progenitor stem cells isolated from adult (7-8 week old) rats then differentiate them into myotubes for experimentation. Any advice would be greatly...

19 September 2012 3,006 4 View

How long should ADSVCs be exposed to differentiation cocktail?

What is the average time, in days, that pre-adipocytes (ie - human adipose derived stromal vascular cells or ADSVC's) should be exposed to the differentiation cocktail (ie- dexamethasone, insulin,...

29 May 2012 1,486 9 View