Damian J Williams

3 Questions 10 Answers 0 Followers

Questions related from Damian J Williams

Any tips for patching CHO cells?

I'm finding it really hard to get a gigaohm seal when recording from CHO cells. Are they very finicky? Is there anything I should know about them? I have no problem patching from cultured neurons...

03 September 2020 2,165 14 View

How can I calculate light intensity at the focal plane of an objective?

I have a fluorescent light source and an objective lens and would like to measure the light intensity at the focal plane. I can measure the light intensity at a different position but need to...

02 April 2018 8,148 4 View

How do you empirically determine the minimum mEPSC amplitude when calculating frequency?

I am trying to calculate mEPSC frequency and the smaller amplitude events fall into the noise. Is there a way of calculating a threshold for EPSC amplitude to determine which events should be...

27 February 2015 5,159 4 View