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RAW 264.7 cells exhibit high response to LPS even in the low concentration of 10ng/ml. And the nitric oxide in the supernatant of cells cocultured with LPS (10ng/ml) after 24H would rise to 60uM...
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I am culturing RAW cells in dishes now. I have split the cells by trypsin before, but the cells' nitric oxide (NO) concentration is very high. So I changed my splitting method using pipetting...
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