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I am trieng to preform a calorimetric TRAP assay in RAW264.7 cells using RANKL for differentiation. I plate 5000c/w in 96w plate using DMEM with 10% FCS as assay medium. Cells at passage 3-15....
01 November 2015 5,722 5 View
Cells were frozen at 10% DMSO, slowly using Ms Frosty. Apn thaw smears and aggregates of cells were observed and the cells could not be extracted from them. Any suggestions?
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