Dalia El-husseini

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Questions related from Dalia El-husseini

Why i do not get a clear solution after breaking the emulsion of emPCR using water saturated diethyl ether? what should i do to improve that?

I'm facing a problem with emulsion PCR, the emulsion phase is made of 95.05% mineral oil, 4.5% span 80, 0.4% tween 80 and 0.05% Triton x-100. For PCR, platinum pfx kit (invitrogen) is used. 50 ul...

11 November 2018 8,117 0 View

What are the specifications of span 80, tween 80, triton x100 and mineral oil to be used in emulsion PCR?

I'm going to perform emulsion PCR using William's method but i'm confused of the specifications of each of the used reagents. for example; there are various types span 80 in sigma aldrich such as:...

12 December 2017 3,291 3 View