Daisy Gates

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Questions related from Daisy Gates

Reduce hemoglobin in RNA extracted from bird blood for Illumina sequencing

We want to sequence RNA from passerine bird blood to look at host expression. We will need to remove the ribosomal RNA and also the globin RNAs during the library preparation. There is no...

17 July 2017 1,931 4 View

TURBO DNase innactivation using Trizol + chloroform+ isopropanol on RNA suspended in n.f water?

I have extracted RNA from avian blood using TRIzol from ambion. After a 3M sodium acetate / ethanol precipitation, the RIN number reads >8 on the bioanalyzer. I want to treat the RNA with TURBO...

18 May 2017 3,191 5 View