How do I estimate protein synthesis in C2C12 myotubes using Puromycin (SUnSET method)?

I have evaluated protein synthesis rate in C2C12 myotubes using SUnSET method. 100 nM insulin or 10 mM Leucine were used as a positive control, but puromycin incorporation did not change in these...

28 March 2022 1,907 0 View