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Currently, I am testing Pst DC3000 cfu assay on Arabidopsis Col-0. DC3000 was grown on KB (Rif) media, and I infected with OD600=0.001 density by hand syringae infiltration. 0 day cfu result...
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Transiently effective VIGS using TRV is a strong technique for functional genomics in plants. Even pTRV1 and pTRV2 empty vector transiently expressed in planta, systemic leaves often show curling...
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