Why my protein purification from 293F supernatant by Ni-NTA but cannot elute from the beads?

I'm trying to express recombinant protein from mammalian cell 293F by its secretion in to the supernatant and then using the Ni-beads to enrich the His-tag protein. However, when I use 300mM...

11 July 2019 4,657 5 View