Czuee Morey

3 Questions 4 Answers 0 Followers

Questions related from Czuee Morey

CHIP-seq using recombinant protein?

I'm from the protein field, and I'm trying to gain more knowledge about genomics technologies. So this might be a very stupid question. I read that in CHIP-seq people use antibodies to detect the...

03 May 2016 6,981 2 View

Can there be duplicate genes on different chromosomes (not through transposition)? What are they called?

I work with a set of 3 conserved proteins that have similar function, very similar structures and about 50% sequence identity. The genes for these proteins are, however, present on different...

16 September 2014 9,821 10 View

Do you know of any tricks to solubilize protein that precipitates in absence of urea?

I am trying to purify a protein with pI 6.4. I use Tris buffer at pH 8, 500mM NaCl for the purification. For homologs of the protein, I have a standardized protocol, where I use urea during lysis,...

16 December 2013 4,305 4 View