Do you know of any tricks to solubilize protein that precipitates in absence of urea?

I am trying to purify a protein with pI 6.4. I use Tris buffer at pH 8, 500mM NaCl for the purification. For homologs of the protein, I have a standardized protocol, where I use urea during lysis,...

16 December 2013 4,266 4 View