Cynthia Martinez

2 Questions 1 Answers 0 Followers

Questions related from Cynthia Martinez

Why in hemolytic assays OD is measured at 490 nm? What is measured at that absorbance?

In the hemolysis assays after incubating the erythrocytes with my protein, centrifuged to remove the cells and measured the absorbance of the supernatant at 490 nm (according to the protocol) but,...

04 July 2017 2,721 7 View

How I can quantify the concentration of primers purified by polyacrylamide gels?

I did the protocol, but when quantifying said measuring at 260 nm the first purified and multiply by 3.3, which is the extinction coefficient however, I have as a control the concentration of the...

20 May 2016 9,287 3 View