Cynthia Bessem Nkemeto

3 Questions 11 Answers 0 Followers

Questions related from Cynthia Bessem Nkemeto

What is the recommended wavelength to perform thermal unfolding of alpha-helical proteins by CD?

Hello, I intend to perform thermal unfolding of my proteins by CD, to determine their melting temperatures. I initially scanned the proteins over a wavelength range of 250 -190 nm at 25 °C (in...

01 June 2022 121 5 View

Protocol for maleimide-PEG coupling to a protein containing a single cysteine residue?

Hello, does anyone have a suitable protocol for coupling maleimide-PEG to a protein? The protein has just one cysteine residue (which was genetically engineered), MW is ~ 6.7 kDa, and...

29 July 2020 3,027 4 View

How to analyze variable temperature CD data for peptides to determine Tm, dH, and dG?

I am having trouble calculating free energy of folding, enthalpy and heat capacity of my peptide samples. I performed a thermal unfolding of the peptides, and obtained the ellipticity in mdeg....

10 October 2019 9,378 6 View