Cuc Hoang Do

4 Questions 5 Answers 0 Followers

Questions related from Cuc Hoang Do

Can anyone show me the ratio concentration of COT1 DNA and DNA labeling in fluorescence of in situ hybridization assay?

I have labelled the probes with bacterial artificial chromosome (BAC) DNA ( from RP11 library). I used it to do Fluorescence in situ hybridization (FISH), but I don't know how much COT 1 DNA I...

20 May 2015 275 0 View

How can I purchase RPCI-11 BAC DNA contigs which cover 17p13.1 band?

I would like to buy nearly 20 Bacterial Artificial Chromosomes DNA contigs of RPCI 11 library. Then, I label these DNA to make FISH probes, so I want to buy DNA , not store in plasmids. Does...

30 March 2015 3,748 2 View

Any advice on Fluorescent in situ hybridization in suspension (FISH-IS) with Locus- specific probes (LSI) ?

I am struggling with detecting 17p deletion in Chronic lymphocytic leukemia (CLL) by FISH - IS using BAC ( bacterial artificial chromosome) with ImageStream X (Amnis), otherwise centro-mere probes...

03 March 2015 1,588 7 View

Does anyone have experience with nick translation to make a probes? Why are commercial probes ( e.g. Vysis probe ) brighter and long lasting?

See above

23 August 2014 5,851 1 View