Crystina Kriss

4 Questions 7 Answers 0 Followers

Questions related from Crystina Kriss

Will using a dounce homogenization in PBS lyse hepatocytes to leave only nuclei or just create a single cell suspension?

I am trying to streamline a nuclear fractionation from liver tissue and was having great luck using a nylon mesh strainer to grind the tissue into a single cell suspension before beginning....

11 April 2017 5,496 2 View

Can you help me with IP questions regarding same species/antibody cross-reactivity?

I am new to IP and understand the basics but now a question has arisen that I am having trouble comprehending. I am using mouse liver homogenate that I have isolated nuclear extracts from. I...

30 April 2015 3,325 4 View

Everytime I run human liver samples on our mass spec they clog our column.

Everytime I run human liver samples, cytosolic and nuclear fractions, on our mass spec they clog our column. I use sodium deoxycholate to solubilize membrane proteins, along with FASP & desalt...

02 March 2015 6,265 9 View

Can someone please supply me with the concentrations/protocol for TCA and HCL-Acetone for washing histones after acid extraction?

I am currently working on histone modifications and am following the abcam protocol for the acid extraction.

29 April 2014 4,573 2 View