Multiplexed assay using Taqman probes gets inconsistent non-exponential amplifications on technical replicates, does anyone have this same issue ?

I'm using Taqman probes to run a Multiplex assay with 5 pairs of primers & 5 probes, and I'm getting these non-exponential amplifications in early cycles (Ct 2-9) for one of 2 technical...

12 April 2022 8,790 11 View