Cristina Torres-Fuentes

4 Questions 24 Answers 0 Followers

Questions related from Cristina Torres-Fuentes

Mortality rate for BL6C57 8 weeks female mice in 3% DSS colitis model?

Hi all, I would like to know if someone has ever done DSS (3%) colitis in BL6C57 8-9 weeks old female mice? I am seeing quite variable results between different cohorts and in the last cohort I...

09 March 2018 9,238 4 View

Does anyone know some minimal media for growing Lactobacillus and Bifs?

I need to test their supernatant on my mammalian cell culture but it interferes with my assay. I need some media with really low Carbon source but still enough to allow the bacteria to survive...

10 April 2014 1,034 12 View

Does anyone have a suggestion for bacteria lysis by sonication?

I have to do some screenings with Lactobacillus and Bifs in mammalian cells. I will carry out the study of the cell lysate. I think, the most easy way is sonication. Which power/time/number of...

10 April 2014 5,163 13 View

Can anyone help with bacteria and mammalian cells co-culture?

I have to study some effects of probiotic bacteria on HEK-cells. In particular, I have to analyse their modulation activity on a receptor which my cells are overexpressing. I have checked in the...

21 March 2014 2,328 24 View