Cristina Rodriguez

3 Questions 11 Answers 0 Followers

Questions related from Cristina Rodriguez

How can I identify where my transgene is inserted ?

I have a transgenic mouse generated using the CAGGS promoter without directing to a specific locus and I would like to know the transgene insertion site. Thanks

24 November 2016 1,953 7 View

Can anyone indicate me the optimal transfection conditions (ratio lentiviral vector (plvx):MD2.G:psPAX2 and transfection reagent) in HEK cells?

We will try with Fugene HD and Fugene 6 but perhaps other liposomes function better.

17 September 2013 6,652 0 View

Does anyone have problems with the "Lenti-XT HTX Packaging System" from Clontech?

We have previously successfully infected primary cells with the "Lenti-XT HTX Packaging System", but recently Clontech has modified the kit and introduced changes in the packaging mix. Although...

19 June 2013 2,516 13 View