Cristina Caldari

2 Questions 1 Answers 0 Followers

Questions related from Cristina Caldari

Extraction of intracellular protein with TRI reagent for ELISA?

I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...

28 May 2024 7,987 1 View

I am having issues with the LPS I ordered from Sigma (L-2630). Does anyone store this in "silanized tubes" to reduce binding?

I am stimulating murine macrophages (RAW 2264.7 cells) with doses of LPS ranging from 0.1 ug/ml to 100 ug/ml (for 24 h) but not seeing any IL-6 production at all (as measured with an ELISA). I ran...

06 June 2018 7,907 4 View