Cristiane dos Santos Costa

5 Questions 7 Answers 0 Followers

Questions related from Cristiane dos Santos Costa

Is it necessary to adjust the pH of a solution prepared with DMSO and PBS for cell culture?

I'm working with the SH-SY5Y cell line and I need to treat them with rutin. The best way I have found to solubilize this flavonoid is with DMSO 100%, but since it's toxic for the cells, I've...

05 February 2022 7,594 4 View

Why am I having so much trouble finding a positive control for my citoprotection assays?

I've been trying to evaluate the citoprotective potential of lyophilized ayahuasca against hydrogen peroxide induced damage in SH-SY5Y cells, but I can't find a positive control for the...

23 February 2020 4,962 0 View

Should I make the MTT standard curve using a known citotoxic chemical?

I want to evaluate the citotoxicity of a plant extract in SH-SY5Y cells. For my experiments, I'll incubate the cells with different concentrations of the plant exctract for 24h and then I'll...

13 June 2019 6,357 3 View

How much FBS should I add to the media for SH-SY5Y cells?

I'll work with SH-SY5Y cells to evaluate the citotoxicity of a plant extract using the MTT assay to assess cell viability. I know that the cells need more FBS (20-30%) to recover from freezing,...

10 June 2019 9,886 4 View

How can I keep an extract sterile in order to test it in cell cultures?

I've been having trouble while preparing the protocols for my assays. I want to work with undifferentiated SH-SY5Y cells to check the neurotoxicity of a plant extract. I'll use the freeze-dried...

25 May 2019 8,686 5 View