What is a tolerable protein concentration for injection on a C-18 HPLC column?

Dear All, I am working on HPLC separation of catecholamines with a C-18 column (waters atlantis T3, C-18, 5μm, 4.6x50mm). The mobile phase is Water, Acetonitrile (0 to 25% gradient), all buffered...

11 November 2017 8,607 3 View