Cristian Ricaurte

3 Questions 1 Answers 0 Followers

Questions related from Cristian Ricaurte

Why I Do Not have colonies after BP Reaction in gateway cloning?

Hi All, I am trying to get some colonies after the bp reaction but I have been trying this for a while and at this point I have no colonies. I am using the pDONR 221, the fragment is 2.3 kb and...

15 October 2021 1,582 1 View

It is possible to contaminate a conventional PCR with a positive Mycoplasma sample ?

I'm running a conventional PCR in order to detect Mycoplasma spp, however i'm having some problems as some negative samples sometimes amplified and I see the band in the gel and sometimes I do not...

11 December 2018 8,733 1 View

Do you know how can I improve the purity in my DNA/RNA Extractions ?

I'm making nucleic acid extraction with Maxwell RSC equipment. But the purity 260/280 is not too high. I extract the nucleic acids from plasma samples. So I'd like to know if there's anyway to...

13 March 2018 5,158 4 View