Cris Martinez

3 Questions 16 Answers 0 Followers

Questions related from Cris Martinez

Best way to transfect a plasmid into MRC-9 cells?

I am working with a new cell line, MRC9s lung fibroblasts, and for RNAi experiments Lipofectamine RNAimax works great! BUT for transfecting plasmid DNA Lipofectamine 3000 (low and high volumes as...

04 April 2018 1,175 2 View

Why does the antibody for protein (not the tag) does not recognize the tagged protein?

I transfected a couple of eukaryotic cell lines with a vector expressing Protein X with a 3X FLAG tag at both N or C terminus. I have checked that I have the correct sequence, correct reading...

05 May 2014 3,490 15 View

How do you isolate human tRNA(s) for EMSA experiments?

I am trying to show in vitro that my protein can bind tRNA (as a control). The protein is of human origin and I think it would be best to use human tRNAs. Currently it is hard to order human tRNAs...

04 April 2014 9,408 4 View