Costansia Bureta

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Questions related from Costansia Bureta

Following SNL on mice, has any one had difficulties in staining Astrocytes using GFAP? IHC-F?

After SNL i harvested the spinal cord segments, Perfusion fixation with 4% PFA, harvested the spinal segment, overnight in PFA, followed by 10,15,20% sucrose. cryopreserved the sections. cut 30um...

22 January 2018 6,103 4 View

Following SNL on mice, has anyone had any difficulties in detecting/staining Microglia?

After SNL i harvested the spinal cord segments, Using Trizol reagent i isolated RNA then Protein extraction, measured protein concentration by BCA and had a relatively good concentration. Used the...

28 December 2017 1,917 6 View