Following SNL on mice, has any one had difficulties in staining Astrocytes using GFAP? IHC-F?

After SNL i harvested the spinal cord segments, Perfusion fixation with 4% PFA, harvested the spinal segment, overnight in PFA, followed by 10,15,20% sucrose. cryopreserved the sections. cut 30um...

22 January 2018 6,101 4 View