Cory Antonio Ocasio

6 Questions 7 Answers 0 Followers

Questions related from Cory Antonio Ocasio

What is the reactivity of hydroxylamine vs. beta-mercaptoethanol?

After performing protein S-acylation assays, I generally process protein samples after lysis by adding SDS-loading buffer with beta-mercaptoethanol. I was always weary of this as I thought the...

01 May 2019 7,420 1 View

Help with expression of a His10-GFP-3C-protein in P. pastoris problem?

Hello All, I am expressing a recombination protein containing a His10-GFP tag connected to my protein of interest via a 3c cleavage site. The idea is to pull down with talon resin, elute with...

29 March 2019 8,785 2 View

What is the maximum length of the spacer region in a Gibson assembly primer?

How many nucleotides can be placed between the overlap and annealing section of the final reverse primer for NEB assembly (Gibson assembly)? P/T2A ok? This would be the last reverse primer that...

01 November 2017 4,898 3 View

I am wondering what would be the best way to identify novel drug resistant mechanisms?

I am planning an experiment whereby I treat cancer cells with increasing amounts of drug over many passages. I aim to identify cells that ultimately become resistant to the drug at concentrations...

08 March 2017 8,452 3 View

What is the best way to introduce a plasmid and siRNA at the same time if possible?

The plasmid is siRNA resistant, and I'd like to show a rescue in cell cycle phenotype, so it is important to have plasmid expressed first or very soon after si tfx.

21 November 2013 4,396 10 View

Using a nuclear hormone receptor system in yeast. (i. e. ERalpha) to select for strains that prevent its membrane association

Hello All, I am trying to develop a screen in yeast whereby an inhibitor of palmitoylation allows ERa to translocate to the nucleus where it transcribes a resistance gene. I'm wondering what your...

01 January 1970 3,471 0 View