Cory Antonio Ocasio

3 Questions 6 Answers 0 Followers

Questions related from Cory Antonio Ocasio

Help with expression of a His10-GFP-3C-protein in P. pastoris problem?

Hello All, I am expressing a recombination protein containing a His10-GFP tag connected to my protein of interest via a 3c cleavage site. The idea is to pull down with talon resin, elute with...

29 March 2019 8,742 2 View

What is the maximum length of the spacer region in a Gibson assembly primer?

How many nucleotides can be placed between the overlap and annealing section of the final reverse primer for NEB assembly (Gibson assembly)? P/T2A ok? This would be the last reverse primer that...

01 November 2017 4,863 3 View

What is the best way to introduce a plasmid and siRNA at the same time if possible?

The plasmid is siRNA resistant, and I'd like to show a rescue in cell cycle phenotype, so it is important to have plasmid expressed first or very soon after si tfx.

21 November 2013 4,368 10 View