I have very low luminescence units using Promega Dual Luciferase Assay, and I'm unsure whether I have low transfection efficiency or something else?

Hello,  I've cloned a 3' UTR of interest into a pGL3 firefly luciferase vector and have transfected it, along with the pRL-TK Renilla luciferase vector control (1:10 ratio) and my miRNA of...

22 July 2016 2,029 5 View