Can anyone help troubleshoooting the differentiation of U-937 Cells with PMA?

I am currently trying to differentiate U-937 cells into a macrophage like phenotype using PMA. I am currently using 800,000 cells in a mL of RPMI-1640 supplemented with 100ug/mL of PMA. After 48...

20 June 2015 7,332 6 View