Corey Heffernan

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Questions related from Corey Heffernan

Does anyone have tips/recommendations for fixation composition [e.g. additives to Karnovsky's] or embedding etc. of mouse sciatic nerve for TEM?

Any optimised approaches to apply to general procedures. Does anyone have tips/recommendations for fixation composition [e.g. additives to Karnovsky's] or embedding etc. of mouse sciatic nerve for...

02 December 2015 500 4 View

Does anyone have a protocol/tips for cross linking whole rat sciatic nerve for subsequent coIP?

Choice of cross linker, time etc?... Preferably with cleavable cross linker...

02 March 2015 3,321 5 View

My cells are sensitive to DMSO >1%. Can anyone suggest alternative diluents/strategies for diluting DSP crosslinker (Thermo) at minimum vol. for coIP?

DSP precipitates from solution containing less than 10-20% DMSO. I need alternatives since my cells are sensitive to such concentrations of DMSO.

23 May 2013 5,620 7 View