Cooper A. Svajda

3 Questions 4 Answers 0 Followers

Questions related from Cooper A. Svajda

I am performing an enzyme assay where the output is NADH absorbance/fluorescence. Why would I see an increase even in my controls?

Hi there, I have been putting together an enzyme assay that measures the conversion of one sugar substrate into another sugar substrate of a reductase, with the goal of coupling enzyme 1's...

14 March 2023 5,088 2 View

Can Plasmid DNA Survive PCR Thermocycling?

Hey there, So I did a Gibson assembly and transformation yesterday and today I saw colonies! I will make a liquid broth and extract DNA to confirm next week, but until then here’s a question: 2...

04 September 2021 4,607 3 View

Problems PCR Amplifying Chloroplast DNA Fragments?

Hello, I am attempting to amplify two fragments of Tobacco Plastid DNA, each of a length of 1.2 kbp. The PCR worked once, two months ago but I have since needed to replicate for further work but...

24 August 2021 3,729 6 View