Constantin Berger

3 Questions 5 Answers 0 Followers

Questions related from Constantin Berger

Does anybody have a protocol for fixation and paraffin embedding of hiPSC derived pancreatic endocrine cells for subsequent staining of insulin?

Dear community, I am working with hiPSC derived pancreatic cell clusters. I am using the Protocol from Rezania et al. 2012 for generation of hormone expressing cell clusters as well as the air...

08 August 2020 5,668 0 View

How can I remove gelatin from glass slides prior to immunofluorescent staining?

Hi, I am staining cryosections of zebrafish brains embedded in gelatin. Right now I have the problem that some gelatin remains inside the ventricles and around the brain after the staining, giving...

18 November 2015 7,802 5 View

How can I avoid/minimize the use of sterile filters?

Hello, I am working on pancreatic differentiation of hiPSCs. Since this requires daily medium change with freshly supplemented media, I have to use sterile filters nearly every day - this bothers...

01 January 1970 495 3 View