Does in-vitro IgE B cell protocol require media change/replenishment/cell splitting during incubation period?

I am treating human PBMCs with IL-4 and anti-CD40 antibody to induce class-switching of B cells in vitro, a protocol widely used in the literature. The incubation period range from 5-7 days (if...

14 September 2024 7,301 1 View