Connor Ott

3 Questions 1 Answers 0 Followers

Questions related from Connor Ott

What are the best steps to purify a native protein from cell culture media?

I have a custom HEK cell line that produces my protein of interest (8 kDa) and I want to purify the protein out of it. From my readings, there are quite a lot of steps and that will not get it to...

28 April 2023 9,042 3 View

Do the binding kinetics differ in an ELISA when binding native sera protein vs recombinant protein?

I am testing various coating concentration of our recombinant antibody in a direct sandwich ELISA. I am testing coating concentrations of 75, 150, and 300 ng/mL with a standard (His-tagged...

21 February 2023 9,750 3 View

What is the best storage concentration and storage solution for an ELISA standard protein?

My his-tagged ELISA standard protein was received in at 5 mg/mL and I have made aliquots of it to reduce freeze thaw cycles. To create the highest standard, I must make a 1:400,000 dilution and...

19 September 2022 2,256 2 View