Connor Behr

2 Questions 4 Answers 0 Followers

Questions related from Connor Behr

Is the original pUC19 sequence reliable enough for restriction free cloning?

I have a standard pUC19 plasmid that I would like to clone GFP, a promoter, and a new ORI into via restriction free cloning. However, I have some concerns about using the original sequence. On...

16 May 2018 6,555 3 View

Is the original pUC19 sequence reliable enough for restriction free cloning?

I have a standard pUC19 plasmid that I would like to clone GFP, a promoter, and a new ORI into via restriction free cloning. However, I have some concerns about using the original sequence. On...

05 May 2018 2,960 4 View