Colt Egelston

2 Questions 11 Answers 0 Followers

Questions related from Colt Egelston

Is co-staining of V alpha 2 and V beta 5 sufficient for identification of T cells from OT-I mice?

I would like to adoptively transfer OT-I T cells to immune-intact mice and examine tissue later by flow cytometry. Unfortunately, our OT-I T cells are CD45.2 and I do not think we can afford...

04 April 2016 3,884 3 View

Does anyone have a protocol for T cell isolation from a spontaneous mouse model of breast cancer, MMTV-PyMT ?

I have tried using Mitlenyi's Gentlemacs with type III collagenase at 37C for about 45 minutes-60 minutes and I am fairly sure I am over-digesting the T cells. After processing to single cell...

04 April 2015 7,501 4 View