Why do my 3T3 cells "atropy" & die almost instantly on changing from abx to abx-free MDEM?

I have 3T3 cells seeded onto 24-well plates with a density of 6000 cells/well in MDEM containing 10% BCS and 1/200 streptomycin-pencillin. The cells were incubated for 72 hours at 37 degrees in 5%...

11 November 2015 3,867 3 View