Colette Giethlen

2 Questions 3 Answers 0 Followers

Questions related from Colette Giethlen

How can I obtain a homozygous gene KO using CRISPR-Cas9 in iPS cells?

I want to generate a KO of my favourite gene using CRISPR-Cas9 in iPS cells. I already obtained an heterozygous clone but I was wondering if I could transfect this clone again to improve chances...

16 October 2015 1,097 3 View

How do I screen for mutations after CRISPR/Cas9 experiments in hiPSC ?

I plan to do a hiPS cell line with the CRISPR/Cas9 technology and wanted to know how to screen mutants after transfection. Because there will be either homozygous or heterozygous events...so...

10 April 2015 5,823 23 View