Cole Peters

2 Questions 10 Answers 0 Followers

Questions related from Cole Peters

What is the origin of replication of pHCMV-VSV-g plasmid?

It's the one from the weinberg lab that everyone uses: https://www.addgene.org/8454/

11 November 2015 4,114 0 View

What protocols are good/best for separating nuclear and cytoplasmic fractions in stem cells or cells with a large nuclei:cytoplasmic ratio?

I've been trying to fractionate glioblastoma stem cells (essentially transformed neural stem cells/progenitor cells) using either this Nature Methods protocol: Analysis of nuclear RNA interference...

09 September 2015 7,031 3 View