Cole Peters

3 Questions 13 Answers 0 Followers

Questions related from Cole Peters

Does anyone have an idea why supplying a gene in trans would differently effect a phenotype than supplying it in cis?

I'm trying to understand why transduction of cells with a viral gene under its viral promoter yields a different phenotype than when the same gene and promoter are incorporated into a viral vector...

29 December 2016 6,059 2 View

What is a good validated antibody for immunoprecipitation?

I'm using a non-denaturing lysis buffer (50mM TrisHCL pH7.5, 150mM NaCl, 1%NP40/IGEPAL +ROCHE protease inhib., 1mM PMSF(fresh), 10mM B-glycerophosphate(fresh)) to lyse cells (30min on ice, then...

05 February 2016 2,439 2 View

Does methylene blue disrupt DNA probe binding to RNA?

I've been trying to perform a Northern blot for a while now, with no positive results. I use Digoxigenin labeled PCR products as a probe (after boiling for 5min) in hybridization buffer...

11 January 2016 3,534 2 View