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I'm using a non-denaturing lysis buffer (50mM TrisHCL pH7.5, 150mM NaCl, 1%NP40/IGEPAL +ROCHE protease inhib., 1mM PMSF(fresh), 10mM B-glycerophosphate(fresh)) to lyse cells (30min on ice, then...
05 February 2016 2,426 2 View
I've been trying to perform a Northern blot for a while now, with no positive results. I use Digoxigenin labeled PCR products as a probe (after boiling for 5min) in hybridization buffer...
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