Why are my M1 macrophages (THP-1) dying after ~5 days post-polarization?

I'm following the protocol (https://www.frontiersin.org/articles/10.3389/fphar.2018.00071/full): Cell differentiation was induced via a 6-h exposure to 185 ng/ml phorbol 12-myristate 13-acetate...

20 April 2022 9,549 3 View