Cody Aros

4 Questions 1 Answers 0 Followers

Questions related from Cody Aros

How can I resolve problems of maintaining tissue integrity during immunofluorescence for paraffin-embedded sections?

Hi, We are performing immunofluorescence on paraffin-embedded sections. We bake at 60°C for 1-2 hours to melt paraffin followed by 2 xylene washes 15 mins each, 100% ethanol 5 mins, 95% ethanol 5...

19 October 2018 3,304 4 View

What is the strength of signal from ROSA mTmG mice in fixed, paraffin-embedded tissue?

I am looking to use ROSA mTmG mice with a cell-specific Cre. I will have tissues fixed in 4% PFA, paraffin-embedded, and sectioned for studies. Is the signal from tdTomato and GFP strong enough...

13 April 2018 2,909 1 View

How can I perform subcellular fractionations for cytoplasm and nuclei while maintaining protein-protein interactions?

I am looking to perform IP/co-IP studies and possibly downstream mass spectrometry applications from protein lysate. Ideally I would IP/co-IP on cytoplasmic and nuclear fractions. However, my...

11 March 2017 6,484 1 View

How can polyclonal antibodies be phospho-specific?

I am studying a tyrosine phosphorylation event on a protein and have both polyclonal and monoclonal antibodies that recognize the phosphorylated residue. My understanding is that polyclonal...

17 November 2016 9,050 3 View