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Hi, We are performing immunofluorescence on paraffin-embedded sections. We bake at 60°C for 1-2 hours to melt paraffin followed by 2 xylene washes 15 mins each, 100% ethanol 5 mins, 95% ethanol 5...
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I am looking to use ROSA mTmG mice with a cell-specific Cre. I will have tissues fixed in 4% PFA, paraffin-embedded, and sectioned for studies. Is the signal from tdTomato and GFP strong enough...
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