3 Questions 1 Answers 0 Followers
Questions related from Cody Aros
Hi, We are performing immunofluorescence on paraffin-embedded sections. We bake at 60°C for 1-2 hours to melt paraffin followed by 2 xylene washes 15 mins each, 100% ethanol 5 mins, 95% ethanol 5...
19 October 2018 3,289 4 View
I am looking to use ROSA mTmG mice with a cell-specific Cre. I will have tissues fixed in 4% PFA, paraffin-embedded, and sectioned for studies. Is the signal from tdTomato and GFP strong enough...
13 April 2018 2,899 1 View
I am looking to perform IP/co-IP studies and possibly downstream mass spectrometry applications from protein lysate. Ideally I would IP/co-IP on cytoplasmic and nuclear fractions. However, my...
11 March 2017 6,472 1 View