Clinton A Oakley

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Questions related from Clinton A Oakley

Proteome search databases - Better results from combining genomes/databases from multiple sources?

We're working on generating proteomics datasets of non-model organisms which show considerable diversity between genera and for which there are several gene model sets available, of varying...

11 November 2018 3,146 3 View

How to reduce high background noise in an LC MS/MS experiment?

We are attempting a shotgun proteomics experiment using LC ESI-MS/MS with an Orbitrap LTQ XL, but are suddenly encountering a problem with excessive background noise. The noise level is fairly...

02 February 2015 4,231 2 View

Is there a substitute formula for NuPage SDS-PAGE Antioxidant reagent?

We are using NuPAGE gels to resolve protein samples, and the included protocol calls for the addition of 0.5mL "antioxidant" to the inner gel chamber. I understand the purpose of the reagent, but...

03 March 2014 5,840 0 View

How can I overcome the dynamic range problem in proteomics of photosynthetic cell culture?

We are attempting non-quantitative shotgun proteomics of unicellular algal culture cell lysates using LC-ESI-MS/MS Orbitrap, but are encountering the issue of only detecting a few dozen very...

03 March 2014 9,851 2 View