How it's possible, in a cloning reaction, to have positive PCR bacterial clones but empty plasmids at the end ?

Hello, For one of my project, I have to clone genomic cDNA fragments (8kb) of SARS-Cov2 virus in a plasmid (5kb, high copy). I amplified 2 cDNA fragments (2 x 4000 pb) through PCR and I used In...

09 March 2021 8,312 6 View