Clay Moran Patton

2 Questions 6 Answers 0 Followers

Questions related from Clay Moran Patton

What could cause my DNA bands to run too high on my electrophoresis gels while using sodium borate buffer?

I just switched over to using a sodium borate buffer for my DNA electrophoresis instead of TBE. I did this because SB is faster, cooler, and cheaper than TBE or TAE. The problem is that my DNA...

07 September 2016 7,658 2 View

During the RNA precipitation step with isopropanol during a TriZol extraction, can glycogen carry contaminants into the RNA pellet?

I have been doing TriZol RNA extractions of zebrafish embryos with and without glycogen as a carrier. I've found that using glycogen during the isopropanol precipitation step increases the size...

16 August 2016 6,911 6 View