Cláudia Oliveira

3 Questions 3 Answers 0 Followers

Questions related from Cláudia Oliveira

How to solubilize ECM proteins from 2D cell cultures?

Hi everyone, I've been decellularizing 2D cell cultures for immunos and LC-MS/MS using 20 mM NH4OH. Basically, I incubate my cells with this solution for 5 min and keep the ECM that is attached...

31 July 2020 3,347 1 View

Does anyone know of a lipid probe already attached to a DOTAP or DOPE that emits in the far red?

Hello everybody, I'm looking for a lipid like DOTAP or DOPE that is already attached to a fluorescent probe that emits in the far red. Another lipid can be good if is a neutral lipid. Thank you...

11 August 2016 6,307 3 View

Why does the shRNA designed has a smaller size than expected?

We designed a shRNA that in the end should have 53bp. However, when we ran on a denaturing polyacrylamide gel, the size was lower than 50. Also it shows a another band, slightly lower than the...

10 April 2016 8,380 2 View